Description
Principle of the Assay: The Human Galectin 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Galectin 1 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human Galectin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Galectin 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Galectin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Galectin 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.